Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 4 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-500.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Biotium TBE Buffer, 5X
5X TBE (Tris-Borate-EDTA) is a concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis. 1X solution: 89 mM Tris,89 mM boric acid, 2 mM EDTA. Store at room temperature.
Chem-Impex International, Inc. 3-N-Morpholinopropanesulfonic acid | 1132-61-2 | MFCD00006183 | 1KG
3-N-Morpholinopropanesulfonic acid, 1132-61-2, MFCD00006183, 1KG
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 250G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 250G
AG Scientific Inc PBS Tablets 100 Tablets
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Bioworld TAE Buffer (25X), 1 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 25X concentration and has a pH of 8.3 ± 0.1. A concentration of 1X contains 0.04M Tris acetate and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Bioworld TAE Buffer (10X), 4 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 10X concentration and is prepared with 18 megohm water. It has a pH of 8.3 ± 0.2. A concentration of 1X contains 0.4M Tris acetate and 0.01M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Bioworld MOPS-SDS Running Buffer (20X), 1 L
MOPS-SDS Running Buffer for Protein Gel electrophoresisSDS running buffer with MOPS and Tris (pH 7.7)20X concentrationAll bioWORLD running buffers are prepared with high quality reagentsSpecifications:Ready to use, just dilute in distilled water. Save time and ensure reproducible results.1X contains: 50mM MOPS, 50mM Tris base, 1mM EDTA, and 0.1% SDS
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt | 71119-22-7 | MFCD00064350 | 100 g
MOPS sodium salt | Purity: ≥99.5% | Mol Wt: 231.25 | 71119-22-7 | MFCD00064350 | 100 g
Enzo Life Sciences Physiological saline (NaCl 0.9%) (endotoxin-free) (sterile) (1.5ml)
Sterile and endotoxin-free physiological saline for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
Quality Biological Inc PBS (1X), pH 7.4 without Calcium and Magnesium, 4 Liters
PBS (phosphate buffered saline) Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Also useful for immunological applications, such as Western Blotting.
Endotoxin and Mycoplasma tested
Without Calcium and Magnesium
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
pH: 7.3 7.5
Osmolality: 300 ± 5% mOsm/Kg H2O
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (10X), pH 7.4, 4 Liters
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Kd Medical Inc TRIS-HCL BUFFER, 1M,PH 7.4, 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 7.4. One 1 liter bottle.